Description
Product Usage Information
Western Blotting: 1:2000
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting
Specificity / Sensitivity
Cyclin A2 (BF683) Mouse Monoclonal Antibody detects endogenous levels of cyclin A2 and does not cross-react with other cyclins or Cyclin A1.
Species Reactivity: Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a recombinant human cyclin A2 protein.
Background
While overcoming the G1/S checkpoint to commence DNA replication requires cyclin E, and traversing the G2/M checkpoint to initiate mitosis requires cyclin B to be present, cyclin A seems to be required for both S-phase and M-phase (1). A number of studies have described the ability of overexpressed cyclin A to accelerate the G1 to S transition, causing DNA replication, and cyclin A antisense DNA can prevent DNA replication (2-4). Cyclin A availability is apparently the rate-limiting step for entry into mitosis, and cyclin A is required for the completion of prophase (5). At late prophase, cyclin A may no longer be necessary as cdc2/cyclinB1 becomes active (5).
Alternate Names
CCN1; CCNA; CCNA2; Cyclin A; cyclin A2; Cyclin-A; Cyclin-A2
