Description
Product Usage Information
Western Blotting: 1:1000
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting
Specificity / Sensitivity
CD101/IgSF2 (F6H5E) Rabbit Monoclonal Antibody recognizes endogenous levels of total CD101/IgSF2 protein.
Species Reactivity: Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the extracellular domain of human CD101/IgSF2 protein.
Background
Cluster of differentiation molecule 101 (CD101), also known as immunoglobulin superfamily member 2 (IgSF2) and cell surface glycoprotein V7, is a type⯠I transmembrane glycoprotein with an extracellular region composed of seven IgV-like domains (1,2). CD101 is expressed on T cells, dendritic cells (DCs), and monocytes (3). On T cells, CD101 expression is induced following T cell receptor (TCR) stimulation and plays an immunoregulatory role, inhibiting proliferation and IL-2 production by blocking Ca2 flux and nuclear factor of activated T cells (NFAT) nuclear translocation (4). Regulatory T cells (Tregs) can express high levels of CD101 and are more suppressive than CD101 Tregs in suppression assays (5). CD101 is highly expressed on activated mucosal tissue-resident memory T cells and may play a role in balancing anti-inflammatory Tregs and pro-inflammatory Th17 cells in mucosal tissue (6). In CD8 T cells, CD101 and TIM-3 expression defines a terminally differentiated state of exhaustion and dysfunction arising from chronic viral infection (7). In cutaneous DCs, triggering CD101 may lead to IL-10 production and the suppression of T cell proliferation (8). CD101 has been identified as a prognostic marker in glioma correlating with unfavorable overall survival and was found to be expressed at high levels on M2-like tumor-associated macrophages (TAMs) (9).
Alternate Names
CD101; CD101 molecule; Cell surface glycoprotein V7; EWI-101; EWI101; Glu-Trp-Ile EWI motif-containing protein 101; IGSF2; Immunoglobulin superfamily member 2; immunoglobulin superfamily, member 2; leukocyte surface protein; V7
