Description
Product Usage Information
Western Blotting: 1:1000
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting
Specificity / Sensitivity
Chronophin/PDXP (C85E3) Rabbit Monoclonal Antibody detects endogenous levels of total chronophin/PDXP protein.
Species Reactivity: Human, Mouse, Rat, Hamster, Monkey, Bovine
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant mouse MBP-chronophin.
Background
Cofilin is a conserved actin-severing protein required for processes that rely on actin dynamics, including cytokinesis and cell motility (reviewed in 1). Regulation of actin dynamics requires the controlled cycling between the phosphorylated and unphosphorylated forms of cofilin (2). The severing activity of cofilin is inhibited by LIMK or TESK phosphorylation at the conserved amino-terminal Ser3 of cofilin (3,4). Slingshot (SSH) phosphatase, for which there have been three mammalian isoforms identified, dephosphorylates cofilin (5). Chronophin (CIN, PDXP) is a haloacid dehalogenase phosphatase that also dephosphorylates cofilin. Alteration of CIN activity through overexpression of either the wildtype or phosphatase-inactive mutant CIN interferes with actin dynamics, cell morphology and cytokinesis (6).
Alternate Names
Chronophin; CIN; dJ37E16.5; FLJ32703; PDXP; PLP; PLP phosphatase; PLPP; pyridoxal (pyridoxine, vitamin B6) phosphatase; pyridoxal phosphatase; Pyridoxal phosphate phosphatase; testicular secretory protein Li 36
