Description
Product Usage Information
Western Blotting: 1:1000
Immunohistochemistry (Paraffin): 1:25
Immunofluorescence (Immunocytochemistry): 1:25
Flow Cytometry (Fixed/Permeabilized): 1:25
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 94309 .
Protocol
Available protocols: Western Blotting, Immunohistochemistry (Paraffin), Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed/Permeabilized)
Specificity / Sensitivity
MEK1/2 (L38C12) Mouse Monoclonal Antibody detects endogenous levels of total MEK1/2 protein.
Species Reactivity: Human, Mouse, Rat, Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with full length MEK1/2 proteins.
Background
MEK1 and MEK2, also called MAPK or Erk kinases, are dual-specificity protein kinases that function in a mitogen activated protein kinase cascade controlling cell growth and differentiation (1-3). Activation of MEK1 and MEK2 occurs through phosphorylation of two serine residues at positions 217 and 221, located in the activation loop of subdomain VIII, by Raf-like molecules. MEK1/2 is activated by a wide variety of growth factors and cytokines and also by membrane depolarization and calcium influx (1-4). Constitutively active forms of MEK1/2 are sufficient for the transformation of NIH/3T3 cells or the differentiation of PC-12 cells (4). MEK activates p44 and p42 MAP kinase by phosphorylating both threonine and tyrosine residues at sites located within the activation loop of kinase subdomain VIII.
Alternate Names
CFC3; CFC4; Dual specificity mitogen-activated protein kinase kinase 1; Dual specificity mitogen-activated protein kinase kinase 2; ERK activator kinase 1; ERK activator kinase 2; FLJ26075; MAP kinase kinase 1; MAP kinase kinase 2; MAP2K1; MAP2K2; MAPK/ERK kinase 1; MAPK/ERK kinase 2; MAPKK 1; MAPKK 2; MAPKK1; MAPKK2; MEK 1; MEK 2; MEK1; MEK2; MEL; mitogen-activated protein kinase kinase 1; mitogen-activated protein kinase kinase 2; mitogen-activated protein kinase kinase 2, p45; MKK1; MKK2; MP2K1; MP2K2; PRKMK1; PRKMK2; protein kinase, mitogen-activated, kinase 1 (MAP kinase kinase 1)
