Description
Product Usage Information
Western Blotting: 1:1000
Immunoprecipitation: 1:50
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting, Immunoprecipitation
Specificity / Sensitivity
USP32 (E8N8G) Rabbit Monoclonal Antibody recognizes endogenous levels of total USP32 protein.
Species Reactivity: Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu217 of human USP32 protein.
Background
Ubiquitin specific protease 32 (USP32) is a membrane-associated deubiquitinating enzyme that regulates cell proliferation and tumor metastasis (1). The gene maps to chromosomal 17q23, a region commonly amplified in breast cancer (1). Downregulation of USP32 inhibits cell proliferation, migration, and invasion in small cell lung cancer (2). In addition, expression of USP32 has been associated with tumorigenesis in gastric and ovarian cancers (3,4). One identified substrate for USP32 is the endosomal GTPase Rab7, a key regulator of endosomal trafficking. Loss of USP32 inhibits late endosome transport and recycling of late endosome cargos (5).
Alternate Names
Deubiquitinating enzyme 32; NY-REN-60; Renal carcinoma antigen NY-REN-60; Ubiquitin carboxyl-terminal hydrolase 32; ubiquitin specific peptidase 32; ubiquitin specific protease 32; Ubiquitin thioesterase 32; ubiquitin thiolesterase 32; Ubiquitin-specific-processing protease 32; UBP32; USP10; USP32
