Description
Product Usage Information
Western Blotting: 1:1000
Immunohistochemistry (Paraffin): 1:400 - 1:1600
Immunofluorescence (Immunocytochemistry): 1:1600 - 1:3200
Flow Cytometry (Fixed/Permeabilized): 1:800 - 1:1600
Flow Cytometry (Live): 1:800 - 1:1600
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting, Immunohistochemistry (Paraffin), Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed/Permeabilized), Flow Cytometry (Live)
Specificity / Sensitivity
TRA-1-60(S) (TRA-1-60(S)) Mouse Monoclonal Antibody detects endogenous levels of the neuraminidase-sensitive TRA-1-60 antigen.
Species Reactivity: Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with human embryonic carcinoma 2102Ep cl.2A6 cells.
Background
TRA-1-60 and TRA-1-81 antibodies detect antigens present on the surface of human stem, teratocarcinoma, and embryonic germ cells (1). TRA-1-60(S) reacts with a neuraminidase sensitive epitope of a proteoglycan (2,3), while TRA-1-81 reacts with a neuraminidase insensitive epitope on the same antigen. Recently this antigen has been proposed to be a form of the protein podocalyxin (4). TRA-1-60 is also detected in the serum of patients with germ cell tumors (5,6).
Alternate Names
GCTM-2 antigen; gp135; Gp200; MGC138240; PC; PCLP; PCLP-1; PCLP1; PDX; Podocalyxin; podocalyxin like; podocalyxin-like; Podocalyxin-like protein 1; PODXL; PODXL1; TRA-1-60 antigen; TRA-1-61 antigen; TRA-1-80 antigen; TRA-1-81 antigen
