Description
Product Usage Information
Western Blotting: 1:1000
Immunoprecipitation: 1:50
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting, Immunoprecipitation
Specificity / Sensitivity
DUSP3/VHR Antibody detects endogenous levels of total DUSP3 protein. This antibody does not cross-react with other dual-specific phosphatases.
Species Reactivity: Human, Mouse, Rat, Monkey
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to human DUSP3. Antibodies are purified by protein A and peptide affinity chromatography.
Background
Dual specificity protein phosphatase 3 (DUSP3), also known as Vaccinia H1-related phosphatase (VHR), is a small dual-specific phosphatase with specificity for MAP kinase ERK1/2 and JNK, but not for p38 MAPK (1,2). Unlike most of the dual-specific phosphatases, which have inducible expression patterns, DUSP3 is constitutively expressed (2). In antigen-stimulated T cells, DUSP3 is phosphorylated by Zap-70 at Tyr138 (3). Tyr138 phosphorylation is required for DUSP3 to downregulate the ERK and JNK pathways (3).
Alternate Names
dual specificity phosphatase 3; Dual specificity protein phosphatase 3; Dual specificity protein phosphatase VHR; DUS3; DUSP3; serine/threonine specific protein phosphatase; Vaccinia H1-related phosphatase; vaccinia virus phosphatase VH1-related; VHR
