Description
Product Usage Information
Western Blotting: 1:1000
Simple Western™: 1:50 - 1:250
Immunoprecipitation: 1:100
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting, Immunoprecipitation
Specificity / Sensitivity
WRN (F8N8Q) Rabbit Monoclonal Antibody recognizes endogenous levels of total WRN protein.
Species Reactivity: Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of human WRN protein.
Background
The RecQ family of DNA and RNA helicases is a family of enzymes that has been shown to be important to genome integrity (1). Members of this family function in several DNA repair processes, including double-strand break repair, homologous recombination, and re-initiation of DNA replication at stalled replication forks (2,3). Mutations in RecQ helicase family members result in syndromes that display varying types of chromosomal abnormalities and overall genomic instability (1). is a member of the RecQ family that has been identified as the gene underlying Werner's syndrome, an autosomal recessive disorder characterized by premature aging and predisposition to cancer (4). Cells from Werner's syndrome patients exhibit genomic instability that is associated with deficient DNA repair activity (5).
Alternate Names
Bifunctional 3'-5' exonuclease/ATP-dependent helicase WRN; DKFZp686C2056; DNA helicase, RecQ-like type 3; Exonuclease WRN; RecQ protein-like 2; RECQ3; RECQL2; RECQL3; Werner syndrome ATP-dependent helicase; Werner syndrome RecQ like helicase; Werner syndrome, RecQ helicase-like; WRN; WRN RecQ like helicase
