Description
Product Usage Information
Western Blotting: 1:1000
Simple Western™: 1:10 - 1:50
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting
Specificity / Sensitivity
IFIT3 (E8G5Y) Rabbit Monoclonal Antibody recognizes endogenous levels of total IFIT3 protein.
Species Reactivity: Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro353 of human IFIT3 protein.
Background
IFIT3 (Interferon-induced protein with tetratricopeptide repeats 3; Retinoic acid-induced gene G protein (RIG-G)) is part of the IFIT family of interferon-stimulating genes known for their antiviral properties (1,2). IFIT3 is widely expressed in T cells, B cells, plasmacytoid and myeloid dendritic cells, neurons, and kidney cells (3,4). In response to RNA viral infection, IFIT3 regulates IRF3 activation through interaction with TBK1 and MAVS on the mitochondrion (5). IFIT3 also interacts with IFIT1, leading to inhibitory activity against viral RNA lacking the 2â²-O-methylation in the cap structure (6).
Alternate Names
CIG-49; cig41; CIG49; GARG-49; IFI-60K; IFI60; IFIT-3; IFIT-4; IFIT3; IFIT4; interferon induced protein with tetratricopeptide repeats 3; Interferon-induced 60 kDa protein; Interferon-induced protein with tetratricopeptide repeats 3; Interferon-induced protein with tetratricopeptide repeats 4; IRG2; ISG-60; ISG60; P60; Retinoic acid-induced gene G protein; RIG-G
