Description
Product Usage Information
Immunofluorescence (Frozen): 1:50 - 1:200
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Immunofluorescence (Frozen)
Specificity / Sensitivity
P2Y12 (E9J1J) Feline Chimeric Monoclonal Antibody recognizes endogenous levels of total P2Y12 protein.
Species Reactivity: Mouse
Source / Purification
This recombinant chimeric antibody is engineered from P2Y12 (E9J1J) Rabbit mAb #69766 according to animal-free protocols. The chimeric antibody retains its antigen-binding Fab regions from the original rabbit monoclonal antibody but contains a feline-derived Fc domain. When multiplexing, Fc-directed rabbit secondaries are required to detect rabbit-host primary antibodies. The parent antibody, P2Y12 (E9J1J) Rabbit mAb #69766, is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro173 of mouse P2Y12 protein.
Background
Purinergic signaling involves cell signaling pathways that are activated by nucleosides and nucleotides via specific purinergic receptors (1). Purinergic receptors include two large families, P1 receptors that are activated by nucleosides and P2 receptors that are activated by nucleotides. P2 receptors are further divided into ionotropic P2X receptors and metabotropic P2Y receptors. P2Y purinoceptor 12 (P2Y12) is a member of the P2Y receptor family (2). As G protein-coupled receptors, P2Y12 are activated by ADP and ATP to drive downstream second messenger pathways. P2Y12 is required for normal platelet aggregation and blood coagulation (3). In addition to their role in thrombosis, P2Y12 receptors drive purinergic signaling in the central nervous system (CNS). P2Y12 receptors are enriched in microglia and regulate nucleotide-dependent microglia chemotaxis and morphology (4). Furthermore, P2Y12 receptors regulate specialized microglia/neuron junctions that provide microglia-dependent protection of neuronal functions in response to brain injury and disease (5).
Alternate Names
2900079b22rik; 4921504d23rik; p2ry12; P2Y purinoceptor 12; P2Y1; p2y12; purinergic receptor p2y, g-protein coupled 12; purinergic receptor p2y12
