Description
Product Usage Information
Western Blotting: 1:1000
Flow Cytometry (Fixed/Permeabilized): 1:100
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting, Flow Cytometry (Fixed/Permeabilized)
Specificity / Sensitivity
Cox1 Antibody Antibody detects endogenous levels of total Cox1 protein.
Species Reactivity: Human, Mouse, Rat
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the C-terminus of mouse Cox1. Antibodies are purified by peptide affinity chromatography.
Background
Cyclooxygenase1 (Cox1) and cyclooxygenase2 (Cox2), family members with 60% homology in humans, catalyze prostaglandin production from arachidonic acid (1,2). While Cox1 expression is constitutive in most tissues, Cox2 expression is induced by lipopolysaccharide (LPS) and peptidoglycan (PGN) (3). PGN activates Ras, leading to phosphorylation of Raf at Ser338 and Erk1/2 at Tyr204. The activation of MAP kinase signaling results in subsequent activation of IKKα/β, phosphorylation of IκBα at Ser32/36, and NF-κB activation. Finally, activation of the transcription factor NF-κB is responsible for the induction of Cox2 expression (4). Investigators have shown that LPS and PGN induce the clinical manifestations of arthritis and bacterial infections, such as inflammation, fever, and septic shock (5). Research studies have indicated that Cox1 and Cox2 may also play a role in the neuropathology of Alzheimer's disease by potentiating γ-secretase activity and β-amyloid generation (6).
Alternate Names
COX-1; COX1; COX3; cyclooxygenase-1; Cyclooxygenase-1; PCOX1; PES-1; PGG/HS; PGH synthase 1; PGH1; PGHS-1; PGHS1; PHS 1; PHS1; Prostaglandin G/H synthase 1; prostaglandin G/H synthase and cyclooxygenase; Prostaglandin H2 synthase 1; Prostaglandin-endoperoxide synthase 1; prostaglandin-endoperoxide synthase 1 (prostaglandin G/H synthase and cyclooxygenase); PTGHS; PTGS1; RP11-542K23.6
