Description
Protocol
Available protocols: Functional
Specificity / Sensitivity
Biotinylated G4S Linker (E7O2V) Rabbit Monoclonal Antibody recognizes exogenously expressed levels of scFv-based CARs containing a G4S linker.
Species Reactivity: All Species Expected
Background
Magnetic bead-based immunoaffinity cell enrichment is a gentle purification method that can be leveraged, in part, to facilitate a robust interrogation of the biology of immune cells that are engineered to express CARs. For example, isolation of rare subsets of CAR positive cells from a complex, heterogeneous population of cells presents the opportunity for extensive downstream analyses using single-cell omics assays. In the context of CAR cell engineering, magnetic bead-based immunoaffinity cell enrichment can also be useful in scenarios when delivery of the CAR transgene is inefficient, resulting in a small population of cells expressing the CAR transgene.
