Description
Product Usage Information
Immunofluorescence (Frozen): 1:50 - 1:200
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Immunofluorescence (Frozen)
Specificity / Sensitivity
MAP2 (D5G1) Mouse Chimeric Monoclonal Antibody recognizes endogenous levels of total MAP2 protein.
Species Reactivity: Mouse, Rat
Source / Purification
This recombinant chimeric antibody is engineered from MAP2 (D5G1) XP Rabbit mAb #8707 according to animal-free protocols. The chimeric antibody retains its antigen-binding Fab regions from the original rabbit monoclonal antibody but contains a mouse-derived Fc domain. When multiplexing, Fc-directed rabbit secondaries are required to detect rabbit-host primary antibodies. The parent antibody, MAP2 (D5G1) XP Rabbit mAb #8707, is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human MAP2 protein.
Background
Microtubule-associated protein 2 (MAP2) is a neuronal phosphoprotein that regulates the structure and stability of microtubules, neuronal morphogenesis, cytoskeleton dynamics, and organelle trafficking in axons and dendrites (1). Multiple MAP2 isoforms are expressed in neurons, including high molecular weight MAP2A and MAP2B (280 and 270 kDa), and low molecular weight MAP2C and MAP2D (70 and 75 kDa). Phosphorylation of MAP2 modulates its association with the cytoskeleton and is developmentally regulated. GSK-3 and p44/42 MAP kinase phosphorylate MAP2 at Ser136, Thr1620, and Thr1623 (2,3). Phosphorylation at Thr1620/1623 by GSK-3 inhibits MAP2 association with microtubules and microtubule stability (3).
Alternate Names
DKFZp686I2148; MAP-2; MAP2; MAP2A; MAP2B; MAP2C; microtubule associated protein 2; Microtubule-associated protein 2; MTAP2
