Description
Product Usage Information
Western Blotting: 1:1000
Immunoprecipitation: 1:50
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting, Immunoprecipitation
Specificity / Sensitivity
COX2/MT-CO2 (E6U9K) Rabbit Monoclonal Antibody recognizes endogenous levels of total COX2/MT-CO2 protein.
Species Reactivity: Human, Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly222 of human COX2/MT-CO2 protein.
Background
The electron transport chain (or respiratory chain) is a series of mitochondrial protein complexes (Complexes I-IV) that utilize coupled reduction-oxidation (redox) reactions to transfer electrons from donor molecules to acceptors. By coupling this electron transport with active proton pumping across the inner mitochondrial membrane, an electrochemical gradient is generated that is used to drive ATP synthesis (1). Complex IV (cytochrome c oxidase) is the terminal complex of the respiratory chain, responsible for transporting electrons from cytochrome c to molecular oxygen. COX2 (cytochrome c oxidase subunit 2), also known as MT-CO2, is one of 14 subunits of cytochrome c oxidase, and one of three subunits (COX1/MT-CO1, COX2/MT-CO2, and COX3/MT-CO3) encoded by the mitochondrial genome (2). These three mitochondrial-encoded subunits form the functional core of cytochrome c oxidase. Research studies have shown that disruptions to COX2/MT-CO2 function can have deleterious health effects. For example, a novel point mutation in the gene was identified as a risk factor for cardiovascular disease (3), whereas a separate point mutation was causally linked to adult onset cerebellar ataxia (4).
Alternate Names
COII; COX2; COXII; Cytochrome c oxidase polypeptide II; Cytochrome c oxidase subunit 2; cytochrome c oxidase subunit II; mitochondrially encoded cytochrome c oxidase II; MT-CO2; MTCO2
