Description
Product Usage Information
Western Blotting: 1:1000
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting
Specificity / Sensitivity
SH2D2A/TSAd (E2V4X) Rabbit Monoclonal Antibody recognizes endogenous levels of total SH2D2A/TSAd protein.
Species Reactivity: Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human SH2D2A/TSAd protein.
Background
The T cell-specific adapter protein (TSAd), encoded by the gene, is an adapter molecule containing an SRC homology 2 (SH2) domain and a proline-rich region (PRR) in addition to several tyrosine phosphorylation sites (1). The protein is predominantly expressed in T cells, NK cells, and endothelial cells (2,3). TSAd is required for T cell polarity establishment, interaction with antigen-presenting cells (4), and maintenance of T regulatory cell (Treg) function (5). The protein modulates T cell receptor (TCR) signaling in activated T cells via interaction with the tyrosine kinases Lck (6) and Itk (7). In endothelial cells, TSAd is required for VEGF-induced angiogenic sprouting through its binding to phosphorylated VEGF Receptor 2 (VEGFR2) at Tyr951 and coupling of VEGFR2 signaling to c-Src activation (8,9).
Alternate Names
F2771; SCAP; SH2 domain containing 2A; SH2 domain protein 2A; SH2 domain-containing adapter protein; SH2 domain-containing protein 2A; SH22A; SH2D2A; T cell specific adapter protein TSAd; T cell-specific adapter protein; T lymphocyte specific adaptor protein; TSAD; VEGF receptor-associated protein; VRAP
