Description
Product Usage Information
Western Blotting: 1:1000
Immunoprecipitation: 1:50
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting, Immunoprecipitation
Specificity / Sensitivity
La Antigen (D19B3) Rabbit Monoclonal Antibody detects endogenous levels of total La Antigen protein.
Species Reactivity: Human, Mouse, Rat, Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu97 of human La Antigen protein.
Background
La antigen is recognized by antibodies in patients with autoimmune disorders such as systemic lupus erythematosus and Sjögren's syndrome (1). La antigen binds to the 5'-noncoding region of poliovirus RNA and is an IRES trans-acting factor (1,2). Depletion of La antigen reduces the function of poliovirus IRES (3). La antigen, when phosphorylated at Ser366, has been shown to associate with nuclear precursor tRNAs and facilitate their processing (4). The nonphosphorylated La antigen interacts with the mRNAs that have 5'-terminal oligopyrimidine (5'TOP) motifs to control protein synthesis (4).
Alternate Names
autoantigen La; La; La autoantigen; La ribonucleoprotein; La ribonucleoprotein domain family, member 3; La/SSB; LARP3; lupus La antigen; Lupus La protein; Sjoegren syndrome type B antigen; Sjogren syndrome antigen B; Sjogren syndrome antigen B (autoantigen La); small RNA binding exonuclease protection factor La; SS-B; SS-B/La protein; SSB
