Description
Product Usage Information
Western Blotting: 1:1000
Simple Western™: 1:10 - 1:50
Immunoprecipitation: 1:100
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting, Immunoprecipitation
Specificity / Sensitivity
Separase (F4R3M) Rabbit Monoclonal Antibody recognizes endogenous levels of total separase protein. This antibody is expected to detect full-length isoform 1 and the N-terminal cleavage fragment. This antibody is not expected to detect the C-terminal cleavage fragment. Based on the peptide antigen sequence, this antibody is expected to recognize isoform 2. This antibody detects an approximately 40 kDa protein of unknown identity in some cell lines.
Species Reactivity: Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Arg1320 of human separase protein.
Background
Caspase-like protein ESPL1 (separase), also known as separin, is a protease critical for chromosome segregation. It plays a role in spindle stabilization and elongation, apoptosis, and DNA repair. Separase cleaves the SCC1/RAD21 subunit of the cohesin complex at the start of anaphase, allowing sister chromatid segregation, an irreversible step in cell division (1). Separase is negatively regulated by securin, which inhibits its proteolytic activity, and by CDK1/cyclin B-dependent phosphorylation. Both securin and cyclin B are degraded via the anaphase-promoting complex/cyclosome (APC/C) (2,3). Dysregulation of separase activity is linked to cancer and genome instability, making it a target for cancer drug discovery (4,5). Separase is overexpressed in human cancer, and overexpression is thought to induce aneuploidy and tumorigenesis (1,6).
Alternate Names
Caspase-like protein ESPL1; ESP1; ESPL1; extra spindle pole bodies 1, separase; extra spindle pole bodies homolog 1; extra spindle pole bodies homolog 1 (S. cerevisiae); extra spindle pole bodies like 1, separase; extra spindle poles like 1; Extra spindle poles-like 1 protein; FLJ46492; KIAA0165; SEPA; Separase; Separin; separin, cysteine protease
