Description
Product Usage Information
Western Blotting: 1:1000
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting
Specificity / Sensitivity
FTSJ3 (E6T9J) Rabbit Monoclonal Antibody recognizes endogenous levels of total FTSJ3 protein.
Species Reactivity: Human, Mouse
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ile651 of human FTSJ3 protein.
Background
FTSJ3 is an RNA 2'-O-methyltransferase originally discovered through its role in processing of 34S pre-rRNA (1). In addition to contributing to RNA stabilization, 2'-O-methylation at the 5'-cap enables the innate immune system to distinguish self from non-self mRNA (2). While some viruses encode their own 2'-O-methyltransferase enabling them to avoid detection by the innate immune system, HIV does not (3,4). However, it was recently demonstrated that FTSJ3 is recruited to HIV RNA resulting in 2'-O-methylation of the HIV RNA genome and evasion of immune detection (5). Cells deficient in FTSJ3 produced virus with less 2'-O-methylation and upregulated type I interferon expression leading to reduced HIV expression and replication (5).
Alternate Names
2'-O-ribose RNA methyltransferase SPB1 homolog; EPCS3; FLJ20062; FtsJ homolog 3; FtsJ homolog 3 (E. coli); FtsJ RNA 2'-O-methyltransferase 3; FtsJ RNA methyltransferase homolog 3; FTSJ3; pre-rRNA 2'-O-ribose RNA methyltransferase FTSJ3; pre-rRNA processing protein FTSJ3; Protein ftsJ homolog 3; Putative rRNA methyltransferase 3; RRMJ3; rRNA (uridine-2'-O-)-methyltransferase 3; SPB1; SPB1 RNA methyltransferase homolog
