Description
Product Usage Information
Western Blotting: 1:1000
Immunohistochemistry (Paraffin): 1:400 - 1:1600
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting, Immunohistochemistry (Paraffin)
Specificity / Sensitivity
GFR alpha1 (F7R3F) Rabbit Monoclonal Antibody recognizes endogenous levels of total GFRα1 protein. Non-specific nuclear staining was observed in human esophagus by immunohistochemistry.
Species Reactivity: Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala381 of human GFRα1 protein.
Background
Glial cell-derived neurotrophic factor (GDNF) plays an important role in the development and maintenance of the central and peripheral nervous system, renal morphogenesis, and spermatogenesis (1). GDNF and the related GDNF family of ligands (GFLs) neurturin, persephin, and artemin bind to the GDNF family receptor alpha (GFRα) proteins, which signal through the rearranged during transfection (RET) receptor tyrosine kinase (2,3). GDNF binds GFRα1, activating signaling cascades that promote neuronal differentiation, plasticity, and survival. In particular, the pro-survival effect of GDNF/GFRα1/RET signaling in dopaminergic neurons has garnered attention in Parkinson's disease (PD) therapeutic research (4,5). GFRα1 can also function in a RET-independent manner, binding and signaling through the neural cell adhesion molecule (NCAM). This interaction has reported roles in neurogenesis, cell migration, and axon regeneration following nerve injury (6-8). GFRα1 expression has been reported to be upregulated in activated astrocytes under pathological conditions as well, suggesting that it may play a role in modulating neuroinflammation (4). In addition, GFRα1 has been implicated in cancer cell progression, metastasis, and treatment resistance. Its potential as a therapeutic target for cancer is under investigation (9-13).
Alternate Names
DKFZp313E1012; DKFZp686J0156; FLJ10561; FLJ31546; GDNF family receptor alpha 1; GDNF family receptor alpha-1; GDNF receptor alpha-1; GDNFR; GDNFR-alpha-1; GDNFRA; GFR-alpha-1; GFRA1; Glial cell line-derived neurotrophic factor receptor alpha; GPI-linked anchor protein; MGC23045; PI-linked cell-surface accessory protein; RET ligand 1; RET1L; RETL1; TGF-beta related neurotrophic factor receptor 1; TGF-beta-related neurotrophic factor receptor 1; TRNR1
