Description
Product Usage Information
Immunofluorescence (Frozen): 1:50 - 1:200
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Immunofluorescence (Frozen)
Specificity / Sensitivity
S100B (E7C3A) Feline Chimeric Monoclonal Antibody recognizes endogenous levels of total S100B protein.
Species Reactivity: Human, Mouse, Rat
Source / Purification
This recombinant chimeric antibody is engineered from S100B (E7C3A) Rabbit mAb #90393 according to animal-free protocols. The chimeric antibody retains its antigen-binding Fab regions from the original rabbit monoclonal antibody but contains a feline-derived Fc domain. When multiplexing, Fc-directed rabbit secondaries are required to detect rabbit-host primary antibodies. The parent antibody, S100B (E7C3A) Rabbit mAb #90393, is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding His86 of human S100B protein.
Background
Despite their relatively small size (8-12 kDa) and uncomplicated architecture, S100 proteins regulate a variety of cellular processes, such as cell growth and motility, cell cycle progression, transcription, and differentiation. To date, 25 members have been identified, including S100A1-S100A18, trichohyalin, filaggrin, repetin, S100P, and S100Z, making it the largest group in the EF-hand, calcium-binding protein family. Interestingly, 14 S100 genes are clustered on human chromosome 1q21, a region of genomic instability. Research studies have demonstrated that significant correlation exists between aberrant S100 protein expression and cancer progression. S100 proteins primarily mediate immune responses in various tissue types but are also involved in neuronal development (1-4). Each S100 monomer bears two EF-hand motifs and can bind up to two molecules of calcium (or other divalent cations in some instances). Structural evidence shows that S100 proteins form antiparallel homo- or heterodimers that coordinate binding partner proximity in a calcium-dependent (and sometimes calcium-independent) manner. Although structurally and functionally similar, individual members show restricted tissue distribution, are localized in specific cellular compartments, and display unique protein binding partners, which suggests that each plays a specific role in various signaling pathways. In addition to an intracellular role, some S100 proteins have been shown to act as receptors for extracellular ligands or are secreted and exhibit cytokine-like activities (1-4). S100B is abundantly expressed in astrocytes and is commonly used as an astrocytic marker in studies of the mammalian CNS. S100B is also expressed in immature and mature myelinating oligodendrocytes that are chondroitin sulfate proteoglycan (NG2)-positive (5).
Alternate Names
NEF; Protein S100-B; S-100 calcium-binding protein, beta chain; S-100 protein beta chain; S-100 protein subunit beta; S100; S100 beta; S100 calcium binding protein B; S100 calcium binding protein, beta (neural); S100 calcium-binding protein B; S100 calcium-binding protein, beta (neural); S100-B; S100B; S100beta
