Description
Product Usage Information
SignalFlex™ conjugates are produced using highly validated Cell Signaling Technology ® primary antibodies and conjugation methods that have been rigorously tested, ensuring high-quality conjugates and lot-to-lot consistency. These conjugates are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. However, they are not tested on specific assays. Optimal dilutions/concentrations should be determined by the end user. When performing flow cytometry, we recommend using an isotype control conjugate at the same concentration as the antibody conjugate.
Storage
Supplied in PBS (pH 7.2), less than 0.1% sodium azide, and 2 mg/mL BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze.
Specificity / Sensitivity
Phospho-LYN (Tyr397)/LCK (Tyr394)/HCK (Tyr411)/BLK (Tyr389) (E5L3D) Rabbit mAb (SignalFlex™ mFluor™ Red 780 Conjugate) detects endogenous levels of LYN, LCK, HCK, and BLK only when phosphorylated at Tyr397, Tyr394, Tyr411, and Tyr389, respectively. It may cross react with overexpressed phosphorylated Src, YES, FYN, and FGR.
Species Reactivity: Human, Mouse
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr397 of human LYN protein.
Background
The Src family of protein tyrosine kinases, which includes Src, Lyn, Fyn, Yes, Lck, Blk, and Hck, are important in the regulation of growth and differentiation of eukaryotic cells (1). Src activity is regulated by tyrosine phosphorylation at two sites, but with opposing effects. While phosphorylation at Tyr416 in the activation loop of the kinase domain upregulates enzyme activity, phosphorylation at Tyr530 in the carboxy-terminal tail by Csk renders the enzyme less active (2).
Alternate Names
ASV; c-SRC; c-syn protooncogene; c-yes; cellular yes-1 protein; FLJ26625; FYN; FYN oncogene related to SRC, FGR, YES; FYN proto-oncogene, Src family tyrosine kinase; HCK; HCK proto-oncogene, Src family tyrosine kinase; Hematopoietic cell kinase; Hemopoietic cell kinase; HsT441; IMD22; JTK8; JTK9; LCK; LCK proto-oncogene, Src family tyrosine kinase; Lck/Yes-related novel protein tyrosine kinase; Leukocyte C-terminal Src kinase; LSK; Lymphocyte cell-specific protein-tyrosine kinase; lymphocyte-specific protein tyrosine kinase; LYN; LYN proto-oncogene, Src family tyrosine kinase; MGC45350; OKT3-induced calcium influx regulator; p53Lyn; p56-LCK; p56(LSTRA) protein-tyrosine kinase; p56lck; p56Lyn; p59-Fyn; p59-HCK/p60-HCK; p59Hck; p60-Src; p61-Yes; p61Hck; pp58lck; pp60c-src; Protein YT16; protein-tyrosine kinase fyn; Proto-oncogene c-Fyn; Proto-oncogene c-Src; Proto-oncogene c-Yes; Proto-oncogene Lck; Proto-oncogene Syn; proto-oncogene tyrosine-protein kinase fyn; proto-oncogene tyrosine-protein kinase LCK; Proto-oncogene tyrosine-protein kinase Src; Proto-oncogene tyrosine-protein kinase Yes; protooncogene SRC, Rous sarcoma; SLK; SRC; SRC proto-oncogene, non-receptor tyrosine kinase; src-like kinase; src/yes-related novel; SRC1; SYN; T cell-specific protein-tyrosine kinase; T-lymphocyte specific protein tyrosine kinase p56lck; THC6; tyrosine kinase p59fyn(T); tyrosine kinase pp60c-src; Tyrosine-protein kinase Fyn; Tyrosine-protein kinase HCK; Tyrosine-protein kinase Lck; Tyrosine-protein kinase Lyn; tyrosine-protein kinase SRC-1; Tyrosine-protein kinase Yes; v-src avian sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog; v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian); v-yes-1 Yamaguchi sarcoma viral oncogene homolog 1; v-yes-1 Yamaguchi sarcoma viral related oncogene homolog; viral oncogene yes-1 homolog 1; Yamaguchi sarcoma oncogene; Yamaguchi sarcoma viral (v-yes-1) related oncogene homolog; YES; YES proto-oncogene 1, Src family tyrosine kinase; YES1; YES1 proto-oncogene, Src family tyrosine kinase; YT16
