Description
Product Usage Information
Western Blotting: 1:1000
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting
Specificity / Sensitivity
Phospho-ATRIP (Ser224) Antibody detects endogenous levels of ATRIP protein only when phosphorylated at Ser224.
Species Reactivity: Human
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic phoshopeptide corresponding to residues surrounding Ser224 of the human ATRIP protein.
Background
In response to genomic stress, the ATR interacting protein (ATRIP) binds and is phosphorylated by the DNA damage-and checkpoint-activated kinase ATR (ataxia-telangiectasia mutated and rad3-related). Both ATR and ATRIP are integral for checkpoint signaling and are critical in the DNA repair response (1-3). Direct interaction between ATRIP and replication protein A (RPA) at RPA-coated, single-stranded DNA results in the recruitment of phosphorylated ATR/ATRIP to stalled replication forks and sites of DNA damage (3). ATR/ATRIP coordinate DNA repair and cell cycle progression in conjunction with key regulatory proteins, such as Rad17 and the 9-1-1 complex (4). ATR associated with ATRIP can also be stimulated by topoisomerase II binding protein (TOPBP1), suggesting that ATRIP may regulate both ATR localization and activity (5). Cyclin dependent kinase 2 (CDK2) may participate in the regulation of DNA damage response and cell cycle control through phosphorylation of ATRIP at Ser224 (6).
Alternate Names
AGS1; ATM and Rad3-related-interacting protein; ATR interacting protein; ATR-interacting protein; ATRIP; DKFZp762J2115; FLJ12343; MGC20625; MGC21482; MGC26740
