Description
Product Usage Information
Western Blotting: 1:1000
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting
Specificity / Sensitivity
SRPK1 (E7J5U) Rabbit Monoclonal Antibody recognizes endogenous levels of total SRPK1 protein.
Species Reactivity: Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Lys377 of human SRPK1 protein.
Background
Serine/arginine-rich protein-specific kinases (SRPKs) constitute a small family of serine/threonine protein kinases that phosphorylate serine residues in sequence regions containing repetitive serine/arginine-rich dipeptides (SR domains) (1). There are three known family members (SRPK1-3), of which SRPK1 and SRPK2 are the most well-studied. Multiple research studies have shown these kinases play important roles in post-transcriptional regulation, notably via phosphorylation of SR-family RNA splicing factors (2). Early studies of SRPK proteins in mice suggested that SRPK1 was expressed ubiquitously, whereas SRPK2 exhibited more tissue-specific expression patterns, while demonstrating conserved substrate specificity (3). SRPK2 has since been implicated in lipid biogenesis, following activation through sequential phosphorylation by mTORC-activated S6K1 and casein kinase 1 (5). Phosphorylation of SRPK2 at Ser494 and Ser497 promotes nuclear translocation of SRPK2, thereby enabling its regulation of mRNA splicing factors involved in lipid biogenesis. Notably, SRPK1 and SRPK2 have both been implicated in the phosphorylation of key viral proteins (5), including the nucleocapsid protein of the SARS and SARS-CoV-2 coronaviruses (6), a modification that may be essential for viral replication.
Alternate Names
Serine/arginine-rich protein-specific kinase 1; serine/arginine-rich splicing factor kinase 1; Serine/threonine-protein kinase SRPK1; SFRS protein kinase 1; SFRSK1; SR protein kinase 1; SR-protein-specific kinase 1; SRPK1; SRSF protein kinase 1
