Description
Product Usage Information
Western Blotting: 1:1000
Simple Western™: 1:10 - 1:50
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting
Specificity / Sensitivity
IL1RAP (E6W2S) Rabbit Monoclonal Antibody recognizes endogenous levels of total IL1RAP protein.
Species Reactivity: Human, Mouse
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the extracellular domain of human IL1RAP protein.
Background
Interleukin-1 receptor accessory protein (IL1RAP/IL1R3) is a type-I transmembrane glycoprotein and component of the IL-1 and IL-33 receptor signaling complexes (1,2). Research studies have demonstrated that IL1RAP expression is required for IL-1-mediated signaling through its ability to recruit IRAK to the IL-1R complex (3-7). In the context of human disease, research studies have shown that IL1RAP is overexpressed on the surface of AML stem cells and associates with c-KIT and FLT3 to mediate pro-proliferative and pro-survival signaling events downstream of these oncogenic receptor tyrosine kinases (8,9). As such, IL1RAP has emerged as a novel immuno-therapeutic target for AML and other malignancies of the myeloid compartment (10,11).
Alternate Names
C3orf13; FLJ37788; IL-1 receptor accessory protein; IL-1R-3; IL-1R3; IL-1RAcP; IL1AP; IL1R3; IL1RAP; interleukin 1 receptor accessory protein; Interleukin-1 receptor 3; Interleukin-1 receptor accessory protein; interleukin-1 receptor accessory protein beta
