Description
Product Usage Information
Western Blotting: 1:1000
Immunoprecipitation: 1:50
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting, Immunoprecipitation
Specificity / Sensitivity
WDR59 (D4Z7A) Rabbit Monoclonal Antibody recognizes endogenous levels of total WDR59 protein.
Species Reactivity: Human, Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding His356 of human WDR59 protein.
Background
The mTORC1 kinase complex plays a critical role in cell growth regulation (1, 2). mTORC1 activity is modulated by cellular environmental factors (e.g., energy levels, growth factors, amino acids) (3, 4), which are mediated through a protein complex known as GATOR (GAP Activity Towards Rags) (5). GATOR is comprised of two protein subcomplexes (GATOR1 and GATOR2), that function in opposing fashion to regulate mTORC1 activity (5). WDR59 was identified as a component of the GATOR2 subcomplex (also containing Mios, WDR24, Seh1L and Sec13), that functions to positively regulate mTORC1 activity (5). Conversely, the GATOR1 subcomplex (containing DEPDC5, Nprl2 and Nprl3), negatively regulates mTORC1 activity, through activation of RagA and RagB GTPases (5).
Alternate Names
CDW12; CDw12 antigen; FLJ12270; FP977; GATOR complex protein WDR59; GATOR2 complex protein WDR59; KIAA1923; MGC11230; p90-120; WD repeat domain 59; WD repeat-containing protein 59; WDR59
