Description
Product Usage Information
The optimal dilution of the anti-species antibody should be determined by the user. However, the final dilutions below should yield acceptable results for the respective applications. Fluorescent western blotting: 1:15000 In-Cell Western: 1:500
Storage
Supplied in 100 mM PBS, pH 7.2, containing 1% BSA and 0.02% sodium azide. Store at 4°C. Protect from light. Do not freeze.
Protocol
Available protocols: Fluorescent Western
Specificity / Sensitivity
Anti-rabbit IgG (H+L) (DyLight
Species Reactivity: Rabbit
Source / Purification
This antibody is prepared from goat antibodies and purified by immunoaffinity chromatography using antigen coupled to agarose beads.
Background
Near infrared anti-species IgG conjugates are ideal for fluorescent western blotting and In-Cell Western. Cell Signaling Technology's strict quality control procedures assure that each conjugate provides optimal specificity and fluorescence. This product has been optimized for use as a secondary antibody in fluorescent western blotting and In-Cell Western™.
