Description
Product Usage Information
Western Blotting: 1:1000
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting
Specificity / Sensitivity
RBM8A (E5Z8T) Rabbit Monoclonal Antibody recognizes endogenous levels of total RBM8A protein.
Species Reactivity: Human, Mouse, Rat, Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val70 of human RBM8A protein.
Background
The exon junction complex (EJC) plays a critical role in splicing, translation, mRNA localization, and nonsense-mediated decay (NMD). It is composed of four core proteins: eukaryotic initiation factor 4A3 (eIF4A3), Mago homolog (MAGOH), RBM8A or Y18, and metastatic lymph node 51 (MLN51) (1-4). The EJC proteins act as a binding platform for auxiliary factors that control mRNA export and NMD (5). RBM8A and MAGOH bind to key NMD factor UPF3B and dock around 20 nucleosides upstream of exon-exon junctions, which helps facilitate proper splicing and NMD functions (6-8). Ribosomes will determine whether NMD factors are needed when encountering a stop codon during translation. If EJCs exist downstream of the stop codon, the ribosome will recruit the SURF complex, and UPF1 phosphorylation will help facilitate the decay of prematurely terminated mRNAs (9,10). The EJC is also involved in the cell cycle as eIF4A3 is phosphorylated at Thr163 by CDK1 and CDK2, which inhibits NMD function and prevents complex formation (11,12). Aberrant RBM8A expression causes cell cycle dysfunction, while low MAGOH expression affects neurological development and proliferation (13,14). EJC members have been implicated in a wide array of diseases and cancers, including hepatocellular carcinoma, glioblastoma, and neurological disorders (15-17).
Alternate Names
binder of OVCA1; Binder of OVCA1-1; BOV-1; BOV-1A; BOV-1B; BOV-1C; C1DELq21.1; DEL1q21.1; MDS014; RBM8; RBM8A; RBM8B; ribonucleoprotein RBM8; Ribonucleoprotein RBM8A; RNA binding motif protein 8A; RNA binding motif protein 8B; RNA-binding motif protein 8A; RNA-binding protein 8A; RNA-binding protein Y14; TAR; Y14; ZNRP; ZRNP1
