Description
Product Usage Information
Western Blotting: 1:1000
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting
Specificity / Sensitivity
Synaptoporin (F7G6I) Rabbit mAb recognizes endogenous levels of total synaptoporin protein.
Species Reactivity: Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of mouse synaptoporin protein.
Background
Synaptoporin, also known as synaptophysin II, has been identified as an isoform of synaptophysin in small synaptic vesicles; it shares 58% homology with synaptophysin (1,2). Synaptoporin and synaptophysin are N-glycosylated membrane proteins that are part of a heteromultimeric complex in synaptic vesicle membranes (2). In general, synaptophysin is more widely distributed in most areas of the nervous system, whereas synaptoporin expression is restricted in some subareas, e.g., forebrain and spinal cord (2-4). After peripheral nerve injury, synaptoporin expression is upregulated in small dorsal root ganglion neurons and appears to play a role in both physiological and neuropathic pain states (5).
Alternate Names
1500003F20Rik; SPO; Synaptoporin; synaptorin; Synpr
