Description
Product Usage Information
Western Blotting: 1:1000
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting
Specificity / Sensitivity
RPL22 (F1J4Y) Rabbit Monoclonal Antibody recognizes endogenous levels of total RPL22 protein. This antibody does not cross-react with RPL22L1 protein.
Species Reactivity: Human, Mouse, Rat, Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ile70 of human RPL22 protein.
Background
RPL22 and RPL22L1 are highly related 60S ribosomal protein paralogs. They both function in protein synthesis and play distinct and sometimes antagonistic roles in hematopoietic stem cell development through p53 (1-3). RPL22 can directly regulate RPL22L1 expression by binding to an internal hairpin on the RPL22L1 mRNA. RPL22 has also been shown to interact with mRNAs for telomerase, histone H1, and EBER1, a noncoding RNA from the Epstein-Barr virus (EBV) (4-6). Both RPL22 and RPL22L1 have been shown to play roles in carcinogenesis. Inactivation of RPL22 can occur in acute lymphoblastic leukemias, which results in increased expression of Lin28B (2). Low expression of RPL22 has also been described in lung and gastric cancers (7,8). RPL22L1 has been implicated in prostate cancer, and its induction is associated with poor prognosis in colorectal cancer (9,10).
Alternate Names
60S ribosomal protein L22; EAP; EBER-associated protein; Epstein-Barr virus small RNA-associated protein; Epstein-Barr-encoded RNA-associated protein; HBP15; HBP15/L22; heparin-binding protein 15; Heparin-binding protein HBp15; L22; Large ribosomal subunit protein eL22; ribosomal protein L22; RL22; RPL22
