Description
Product Usage Information
Western Blotting: 1:1000
Immunoprecipitation: 1:50
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting, Immunoprecipitation
Specificity / Sensitivity
Emerin (D9B3) Rabbit Monoclonal Antibody detects endogenous level of total Emerin protein.
Species Reactivity: Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human Emerin protein.
Background
Emerin is a broadly expressed integral protein of the nuclear inner membrane (1). It contains a LEM domain and binds to several nuclear proteins, such as BAF (barrier-to-autointegration factor) and A- and B-type lamins, which are important in nuclear functions (2-5). Emerin may regulate gene expression through binding to other transcriptional regulators (6,7). Emerin binds to β-catenin and inhibits its nuclear accumulation (8). Recent studies demonstrate that emerin is required for HIV-1 infectivity (9). Mutations in the gene encoding emerin ( ) are a major cause of Emery-Dreifuss muscular dystrophy (EDMD), a disorder characterized by progressive skeletal muscle weakening (10).
Alternate Names
EDMD; EMD; Emerin; LEM domain containing 5; LEMD5; STA
