Description
Product Usage Information
Western Blotting: 1:1000
Immunofluorescence (Immunocytochemistry): 1:100
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting, Immunofluorescence (Immunocytochemistry)
Specificity / Sensitivity
FoxM1 (D12D5) Rabbit Monoclonal Antibody detects endogenous levels of total FoxM1 protein. This antibody is predicted to recognize all three isoforms (FoxM1a, FoxM1b and FoxM1c).
Species Reactivity: Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant protein to human FoxM1b.
Background
Forkhead box M1 (FoxM1) is a forkhead box family transcription factor that regulates a number of genes throughout the cell cycle to help control DNA replication, mitosis, and cell proliferation. FoxM1 expression increases during G1 and S and reaches maximum levels in G2/M (1-3). Nuclear translocation occurs just before entry into G2/M and is associated with FoxM1 phosphorylation (4). Phosphorylation of FoxM1 by MAPK (Ser331, Ser704), Cyclin/Cdk (Ser4, Ser35, Thr600, Thr611, Thr620, Thr627, Ser638), Plk1 (Ser715, Ser724), and Chk2 (Ser376) stabilizes and activates FoxM1 (4-8). Forkhead box M1 is expressed in all embryonic tissues but is restricted to proliferating tissues in adults (9). Research studies show that FoxM1 expression is negatively regulated by p53 (10,11). Upregulation of FoxM1 is associated with many human cancers, including prostate, breast, lung, ovary, colon, pancreas, stomach, bladder, liver, and kidney, and may be associated with p53 mutations in some tumors (11,12). As a result, FoxM1 inhibitors have become a topic of interest for potential cancer therapy (13).
Alternate Names
FKHL16; forkhead box M1; forkhead box M1-D; Forkhead box protein M1; forkhead-like 16; Forkhead-related protein FKHL16; Forkhead, drosophila, homolog-like 16; FOXM1; FOXM1A; FOXM1B; FOXM1C; Hepatocyte nuclear factor 3 forkhead homolog 11; HFH-11; HFH11; HNF-3; HNF-3/fork-head homolog 11; INS-1; M-phase phosphoprotein 2; MPHOSPH2; MPM-2 reactive phosphoprotein 2; MPP-2; MPP2; PIG29; TGT3; Transcription factor Trident; TRIDENT; WIN; Winged-helix factor from INS-1 cells
