Description
Product Usage Information
Western Blotting: 1:1000
Immunoprecipitation: 1:50
IHC Leica Bond: 1:100 - 1:400
Immunohistochemistry (Paraffin): 1:200
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier-free (BSA and azide free) version of this product see product # 47448 .
Protocol
Available protocols: Western Blotting, Immunoprecipitation, IHC Leica Bond, Immunohistochemistry (Paraffin)
Specificity / Sensitivity
TNFRSF8/CD30 (E4L4I) Rabbit Monoclonal Antibody recognizes endogenous levels of total TNFRSF8/CD30 protein. This antibody is predicted to detect CD30V, which is a truncated isoform that consists of the CD30 cytoplasmic domain. Non-specific staining was observed in the epithelium of mouse prostate tissue.
Species Reactivity: Human, Mouse, Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu500 of human TNFRSF8/CD30 protein.
Background
TNFRSF8/CD30 is a type-I transmembrane glycoprotein that is a member of the TNFR superfamily. CD30 is synthesized as a precursor protein that undergoes extensive post-translational modification before becoming embedded in the plasma membrane as a 120-kDa transmembrane protein (1,2). The expression of CD30 is upregulated in activated T cells and may trigger costimulatory signaling pathways upon its engagement (3,4). While its expression is normally restricted to subsets of activated T cells and B cells, CD30 expression is robustly upregulated in hematologic malignancies, such as Hodgkin lymphoma (HL), anaplastic large cell lymphoma (ALCL), and adult T-cell leukemia, thus making it an attractive target for therapeutic intervention (5,6). Research studies have suggested that in certain disease contexts, CD30 recruits TRAF2 and TRAF5 adaptor proteins to drive NF-kappa B activation, aberrant cell growth, and cytokine production (7-9). CD30 signaling is also regulated by TACE-dependent proteolytic cleavage of its ectodomain, which results in reduced CD30L-dependent activation of CD30 cells (10,11).
Alternate Names
CD30; CD30 antigen; CD30L receptor; cytokine receptor CD30; D1S166E; Ki-1; Ki-1 antigen; Lymphocyte activation antigen CD30; TNF receptor superfamily member 8; TNFRSF8; TNR8; Tumor necrosis factor receptor superfamily member 8; tumor necrosis factor receptor superfamily, member 8
