Description
Product Usage Information
Western Blotting: 1:1000
Immunofluorescence (Immunocytochemistry): 1:50
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting, Immunofluorescence (Immunocytochemistry)
Specificity / Sensitivity
Fascin (55k-2) Mouse Monoclonal Antibody recognizes endogenous levels of total fascin protein.
Species Reactivity: Human, Mouse, Rat, Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with fascin protein purified from HeLa cells.
Background
Fascin is a monomeric, globular protein that plays a central role in regulating the structure and function of the cortical actin cytoskeleton (1). Fascin promotes cross-linkage of parallel actin filaments during the formation of cell protrusions (lamellipodia and filopodia), and therefore plays an important role in regulating cell migration (2). It has been reported that fascin may also regulate filopodia formation by a mechanism independent of its actin-bundling functions (3), though less is known about this mechanism of action. Research studies have shown that increased fascin expression is associated with increased motility and invasiveness of neoplastic cells, including breast, colon, prostate, and esophageal squamous cell carcinomas (4-6). Fascin binds to the armadillo-repeat domain of β-catenin and , and has been shown to co-localize with β-catenin and cadherins at the leading edge of migratory cells (7).
Alternate Names
55 kDa actin-bundling protein; actin bundling protein; epididymis secretory sperm binding protein; FAN1; Fascin; fascin 1; fascin actin-bundling protein 1; fascin homolog 1, actin-bundling protein; fascin homolog 1, actin-bundling protein (Strongylocentrotus purpuratus); FLJ38511; FSCN1; HSN; p55; singed-like (fascin homolog, sea urchin); Singed-like protein; Singed, drosophila, homolog-like; SNL
