Description
Product Usage Information
Flow Cytometry (Fixed/Permeabilized): 1:50 - 1:200
Storage
Supplied in PBS (pH 7.2), less than 0.1% sodium azide, and 2 mg/mL BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze.
Protocol
Available protocols: Flow Cytometry (Fixed/Permeabilized)
Specificity / Sensitivity
T-bet/TBX21 (E4I2K) Rabbit Monoclonal Antibody (Alexa Fluor
Species Reactivity: Human, Mouse
Source / Purification
Monoclonal antibody is produced by immunizing animals with a recombinant protein fragment derived from mouse T-bet/TBX21 protein.
Background
The gene family consists of transcription factors characterized by a related DNA-binding domain (T-box) of approximately 200 amino acids (1,2). The genes exhibit diverse temporal and spatial patterns in the developing embryo. Studies have demonstrated members of this family play crucial roles during embryogenesis in a wide range of organisms by regulating cell fate decisions to establish the early body plan and to regulate later processes underlying organogenesis (3-5). Mutations in genes are associated with many developmental defects (6). Recent studies also indicate potential roles in cancer by members of the T-box family (7-9).
Alternate Names
OTTMUSP00000002150; T-bet; T-box 21; T-box protein 21; T-box transcription factor TBX21; T-cell-specific T-box transcription factor T-bet; Tbet; Tbly; Tblym; TBT1; Tbx21; Transcription factor TBLYM
