Description
Product Usage Information
Western Blotting: 1:1000
Immunoprecipitation: 1:50
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting, Immunoprecipitation
Specificity / Sensitivity
The BSP II Antibody detects endogenous levels of total BSP II protein.
Species Reactivity: Human, Mouse, Rat
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human BSP II protein. Antibodies are purified by protein A and peptide affinity chromatography.
Background
Bone sialoprotein II (BSP II) exists as a secreted protein in extracellular matrix of the skeleton (1-2). BSP II is part of the small integrin binding ligand N-linked glycoprotein (SIBLING) family and its role is crucial for cell to matrix adhesion as well as between two active protein complexes (3). Flexible binding capacity of BSP II also allows it to rapidly associate with various proteins, calcium, and mineralized phases of developing bone (3-5). BSP II plays a central role in the initiation of hydroxyapatite microcalcification of breast cancer cells that contributes to the pathogenesis of bone metastasis (4,6,7). Recent evidence suggests that the downregulation of BSP II may lead to the reduced formation of metastatic lesions and bone metastasis in a human breast cancer model (5).
Alternate Names
BNSP; Bone sialoprotein 2; Bone sialoprotein II; BSP; BSP II; BSP-II; Cell-binding sialoprotein; IBSP; integrin binding sialoprotein; Integrin-binding sialoprotein; SIAL; Sialoprotein-2; SP-II
