Description
Product Usage Information
Western Blotting: 1:1000
Immunoprecipitation: 1:100
Immunofluorescence (Immunocytochemistry): 1:50 - 1:200
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Immunocytochemistry)
Specificity / Sensitivity
MYO10 (F3V7U) Rabbit Monoclonal Antibody recognizes endogenous levels of total MYO10 protein.
Species Reactivity: Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the central region of human MYO10 protein.
Background
Myosin family motor proteins drive ATP-dependent actin-based motility in eukaryotic cells and contain a conserved amino-terminal motor domain (1). Unconventional myosin-X (MYO10) binds actin filaments and localizes to filopodia tips. MYO10 regulates cell migration, metastasis, and neuritogenesis, and its expression is upregulated in mouse and human tumors (2). MYO10 was shown to drive tumor progression by inducing genome instability through cGAS/STING inflammatory response (3). Regulation of MYO10 phosphorylation and protein levels in mitosis is critical for spindle formation, genome stability, and sensitivity of cancer cells to Taxol (4).
Alternate Names
FLJ10639; FLJ21066; FLJ22268; FLJ43256; KIAA0799; MGC131988; MYO10; myosin X; Myosin-X; Unconventional myosin-10; Unconventional myosin-X; unconventionnal myosin-X
