Description
Product Usage Information
Western Blotting: 1:1000
Immunofluorescence (Immunocytochemistry): 1:1600 - 1:6400
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting, Immunofluorescence (Immunocytochemistry)
Specificity / Sensitivity
PTN (F7T3F) Rabbit Monoclonal Antibody recognizes endogenous levels of total PTN protein.
Species Reactivity: Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human PTN protein.
Background
Pleiotrophin (PTN) is a heparin-binding, developmentally regulated cytokine that is independently identified by several groups; it is also known as heparin-binding brain mitogen (HBBM), heparin-binding growth factor-8 (HBGF-8), heparin-binding neurotrophic factor (HBNF), heparin-affinity regulatory peptide (HARP), heparin-binding growth-associated molecule (HB-GAM), and osteoblast-specific factor 1 (OSF-1) (1-6). PTN is highly upregulated during embryonic development and early cell differentiation. In adulthood, PTN expression decreases in most tissues except for the bone and the nervous system. It interacts with numerous cell surface proteoglycan and non-proteoglycan receptors, including protein tyrosine phosphatase receptor type Z (PTPRZ), syndecans, nucleolin, neuropilin-1, ALK, and integrin αVβ3 and αMβ2 (reviewed in 7-9). PTN has been associated with biological events that involve cellular proliferation, differentiation, and inflammation, including tissue regeneration, bone repair, hypoxia, tumor growth, and angiogenesis.
Alternate Names
HARP; HB-GAM; HBBM; HBGF-8; HBGF8; HBNF; HBNF-1; HBNF1; heparin affin regulatory protein; heparin binding growth factor 8; Heparin-binding brain mitogen; Heparin-binding growth factor 8; Heparin-binding growth-associated molecule; heparin-binding neurite outgrowth promoting factor; Heparin-binding neurite outgrowth-promoting factor; Heparin-binding neurite outgrowth-promoting factor 1; NEGF1; neurite growth-promoting factor 1; OSF-1; Osteoblast-specific factor 1; Pleiotrophin; pleiotrophin (heparin binding growth factor 8, neurite growth-promoting factor 1); PTN
