Description
Product Usage Information
Western Blotting: 1:1000
Immunoprecipitation: 1:50
Immunofluorescence (Immunocytochemistry): 1:400
Flow Cytometry (Fixed/Permeabilized): 1:50
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed/Permeabilized)
Specificity / Sensitivity
hnRNP A0 (D8A3) Rabbit Monoclonal Antibody recognizes endogenous levels of total hnRNP A0 protein. This antibody does not cross-react with other hnRNP family members.
Species Reactivity: Human, Mouse, Rat, Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu114 of human hnRNP A0 protein.
Background
Heterogeneous nuclear ribonucleoprotein A0 (hnRNP A0) is a member of the hnRNP A/B family of related RNA binding proteins that bind pre-mRNA and are involved in the processing, metabolism, and transport of nuclear pre-mRNA transcripts (1). The A/B subfamily of hnRNP includes A1, A2/B1, A3, and A0. hnRNP A0 is phosphorylated at Ser84 by MAPKAPK-2 in response to LPS treatment in mouse macrophage cells, which might play a key role in stimulating translation of the TNF-α message (2).
Alternate Names
Heterogeneous nuclear ribonucleoprotein A0; hnRNA binding protein; hnRNP A0; HNRNPA0; HNRPA0; ROA0
