Description
Product Usage Information
Western Blotting: 1:1000
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting
Specificity / Sensitivity
PI3 Kinase p101 (D32A5) Rabbit Monoclonal Antibody recognizes endogenous levels of total PI3 Kinase p101 protein. This antibody also detects a strong background band of unknown origin between 50-60 kDa.
Species Reactivity: Human, Mouse
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly508 of human PI3 Kinase p101 protein.
Background
Phosphoinositide 3-kinase (PI3K) catalyzes the production of phosphatidylinositol-3,4,5-triphosphate by phosphorylating phosphatidylinositol (PI), phosphatidylinositol-4-phosphate (PIP), and phosphatidylinositol-4,5-bisphosphate (PIP ). Growth factors and hormones trigger this phosphorylation event, which in turn coordinates cell growth, cell cycle entry, cell migration, and cell survival (1). PTEN reverses this process, and research studies have shown that the PI3K signaling pathway is constitutively activated in human cancers that have loss of function of PTEN (2). PI3Ks are composed of a catalytic subunit (p110) and a regulatory subunit. Various isoforms of the catalytic subunit (p110α, p110β, p110γ, and p110δ) have been isolated, and the regulatory subunits that associate with p110α, p110β, and p110δ are p85α and p85β (3). In contrast, p110γ associates with a p101 regulatory subunit that is unrelated to p85. Furthermore, p110γ is activated by βγ subunits of heterotrimeric G proteins (4). The p101 regulatory subunit binds to Gβγ, released from heterotrimeric G proteins, and recruits bound p110γ catalytic subunit to the plasma membrane, which is required for GPCR-induced PI3Kγ activity (5).
Alternate Names
F730038I15Rik; FOAP-2; p101; p101-PI3K; Phosphatidylinositol 4,5-bisphosphate 3-kinase regulatory subunit; Phosphatidylinositol-4,5-bisphosphate 3-kinase regulatory subunit; Phosphoinositide 3-kinase regulatory subunit 5; phosphoinositide-3-kinase regulatory subunit 5; phosphoinositide-3-kinase, regulatory subunit 5; phosphoinositide-3-kinase, regulatory subunit 5, p101; phosphoinositide-3-kinase, regulatory subunit, polypeptide p101; PI3-kinase p101 subunit; PI3-kinase regulatory subunit 5; PI3R5; PIK3R5; Protein FOAP-2; PtdIns-3-kinase p101; PtdIns-3-kinase regulatory subunit
