Description
Product Usage Information
IHC Leica Bond: 1:200 - 1:800
Immunohistochemistry (Paraffin): 1:50 - 1:200
Immunofluorescence (Immunocytochemistry): 1:200 - 1:800
Storage
Supplied as liquid tissue culture supernatant containing sodium azide as a preservative. Stable for 6 months when stored at 4°C. Do not aliquot the antibody. Note: This product is only available in the format listed above. We are unable to provide this in a carrier free, custom formulation.
Protocol
Available protocols: IHC Leica Bond, Immunohistochemistry (Paraffin), Immunofluorescence (Immunocytochemistry)
Specificity / Sensitivity
Villin-1 (CWWB1) Mouse Monoclonal Antibody recognizes endogenous levels of villin-1 protein.
Species Reactivity: Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a prokaryotic recombinant protein containing amino acids 5-209 of mature villin-1 protein.
Background
Villin is a member of the gelsolin family of calcium-regulated actin-binding proteins. Unlike the ubiquitously expressed gelsolin, villin expression is restricted to simple epithelia of the gastrointestinal and urogenital tracts. It is localized to the apical cytoplasm and brush borders of these cells. Villin functions in the regulation of actin dynamics in the apical epithelium, capping, nucleating and/or severing actin filaments in a calcium-dependent manner and regulating cell shape in response to external stimuli (1,2). Phosphorylation of villin at Tyr60, 81, and 256 may be involved in the regulation of cell migration (3). Expression of villin is increased in colorectal cancers (4), and villin-1 function appears to be involved in progressive cholestasis and hepatic failure (5).
Alternate Names
D2S1471; VIL; VIL1; VILI; Villin; villin 1; Villin-1
