Description
Product Usage Information
Western Blotting: 1:1000
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting
Specificity / Sensitivity
WIF1 (D50F9) Rabbit Monoclonal Antibody detects endogenous levels of total WIF1 protein.
Species Reactivity: Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro168 of human WIF1 protein.
Background
WIF1 (Wnt inhibitory factor 1) is a secreted protein that binds to Wnt proteins and inhibits their activity (1). It contains an N-terminal WIF domain and five EGF-like repeats (2). The WIF1 ortholog in Drosophila, Shifted, is required for Hedgehog stability and diffusion (3,4). It has been reported that WIF1 expression is downregulated in many types of cancers (5-8).
Alternate Names
WIF-1; WIF1; Wnt inhibitory factor 1
