Description
Product Usage Information
Western Blotting: 1:1000
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting
Specificity / Sensitivity
HOP (D6E3) Rabbit Monoclonal Antibody recognizes endogenous levels of total HOP protein. This antibody is not known to cross-react with other heat shock proteins.
Species Reactivity: Human, Mouse, Rat, Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Lys513 of human HOP protein.
Background
HOP, also known as stress-induced phospho protein 1 (STIP), is a co-chaperone to the major heat shock proteins, Hsp70 and Hsp90, and appears in early receptor complexes (1,2). Through mutual binding to both Hsp70 and Hsp90, Hop functions as an adaptor that can integrate Hsp70 and Hsp90 interactions (3,4). HOP is an abundant and highly conserved protein which is composed of three tetratricopeptide repeat (TPR) domains (TPR1, TPR2a and TPR2b) and two DP repeat domains (DP1 and DP2), whose function has not been fully resolved (5).
Alternate Names
epididymis secretory sperm binding protein Li 94n; HEL-S-94n; Hop; Hsc70/Hsp90-organizing protein; Hsp70/Hsp90-organizing protein; IEF-SSP-3521; NY-REN-11 antigen; P60; Renal carcinoma antigen NY-REN-11; STI1; STI1L; STIP1; stress induced phosphoprotein 1; Stress-induced-phosphoprotein 1; stress-induced-phosphoprotein 1 (Hsp70/Hsp90-organizing protein); Transformation-sensitive protein IEF SSP 3521
