Description
Product Usage Information
Simple Western™: 1:10
Flow Cytometry (Live): 1:100 - 1:400
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 66159 .
Protocol
Available protocols: Flow Cytometry (Live)
Specificity / Sensitivity
Whitlow/218 Linker (E3U7Q) Rabbit Monoclonal Antibody recognizes exogenously expressed levels of scFv-based CARs containing a Whitlow/218 linker. This antibody is not approved for traditional Western blot analysis.
Species Reactivity: All Species Expected
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues of the Whitlow/218 linker peptide.
Background
The Whitlow/218 peptide is a commonly used linker that connects the variable heavy (VH) domain and variable light (VL) domain of single-chain variable fragments (scFvs) and chimeric antigen receptors (CARs) that utilize an extracellular domain scFv for target antigen recognition. The Whitlow/218 linker affords the scFv with enhanced resistance to proteolysis and aggregation (1).
