Description
Product Usage Information
Western Blotting: 1:1000
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting
Specificity / Sensitivity
SARS-CoV-2 Nsp1 Antibody recognizes endogenous levels of total SARS-CoV-2 Nsp1 protein in infected cells.
Species Reactivity: Virus
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala76 of SARS-CoV-2 Nsp1 protein. Antibodies are purified by peptide affinity chromatography.
Background
The cause of the COVID-19 pandemic is a novel and highly pathogenic coronavirus, termed SARS-CoV-2 (severe acute respiratory syndrome coronavirus-2). SARS-CoV-2 is a member of the Coronaviridae family of viruses (1). The genome of SARS-CoV-2 is relatively large and encodes up to 29 open reading frames (ORFs). These include ORF1a and ORF1b (further processed into 16 non-structural proteins), 9 accessory proteins, and 4 canonical structural proteins: spike (S), envelope (E), membrane (M), and nucleocapsid (N) (2). Nsp1 is the first protein to be translated from the viral RNA by the eukaryotic translational machinery. It has previously been shown that Nsp1 from SARS-CoV inhibits the host response to viral RNA by inhibiting host cell translation including mRNA encoding Type 1 interferon (3). The ability of SARS-CoV-2 Nsp1 has also been shown to exert inhibition of host cell translation, specifically by binding to the 40S ribosomal subunit, and competing out entry of M G capped eukaryotic mRNA to the entry channel of the pre-initiation translation complex (4,5).
Alternate Names
Host translation inhibitor nsp1; leader protein; non-structural protein 1; non-structural protein 1 (1-180); nsp1; nsp1-pp1a-pp1ab; SARS-CoV-2 nsp1; severe acute respiratory syndrome coronavirus 2 non-structural protein 1
