Description
Product Usage Information
Western Blotting: 1:1000
Simple Western™: 1:10 - 1:50
Immunoprecipitation: 1:200
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting, Immunoprecipitation
Specificity / Sensitivity
NLRP7 (F9U8L) Rabbit Monoclonal Antibody recognizes endogenous levels of total NLRP7 protein.
Species Reactivity: Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Glu104 of human NLRP7 protein.
Background
NOD-like receptor (NLR) family pyrin domain containing 7 (NLRP7) is a cytoplasmic immune receptor protein that recognizes microbial lipopeptides (1). NLRP7 is expressed in primates, but rodents have no orthologous genes (2-4). NLRP7 is required for IL-1β release in response to spp. infection, and is activated in response to infection with other types of Gram-positive bacteria (1). While the mechanism is not well understood, NLRP7 is also critical for placental development; mutations in maternal may result in hydatidiform mole, a type of abnormal pregnancy that results in spontaneous abortion, defective embryonic development, or reproductive wastage (5,6). Mutations in males, however, do not negatively impact reproductive outcomes (6). NLRP7 may also play a role in gut homeostasis, as a particular coding variant, p.S361L, is associated with increased risk of developing ulcerative colitis (7).
Alternate Names
CLR19.4; FLJ94610; HYDM; MGC126470; MGC126471; NACHT, leucine rich repeat and PYD containing 7; NACHT, LRR and PYD containing protein 7; NACHT, LRR and PYD domains-containing protein 7; NALP7; NLR family pyrin domain containing 7; NLR family, pyrin domain containing 7; NLRP7; NOD12; Nucleotide-binding oligomerization domain protein 12; nucleotide-binding oligomerization domain, leucine rich repeat and pyrin domain containing 7; PAN7; PYPAF3; PYRIN-containing APAF1-like protein 3
