Description
Product Usage Information
Western Blotting: 1:1000
Immunoprecipitation: 1:200
Flow Cytometry (Live): 1:200
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting, Immunoprecipitation, Flow Cytometry (Live)
Specificity / Sensitivity
CA12 (D75C6) Rabbit Monoclonal Antibody detects endogenous levels of total CA12 protein.
Species Reactivity: Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly39 of human CA12 protein.
Background
Carbonic anhydrases (CA) are a family of ancient zinc metalloenzymes found in almost all living organisms. All CA can be divided into 3 distinct classes (α, β, and γ) that evolved independently and have no significant homology in sequence and overall folding. All functional CA catalyze the reversible hydration of CO into HCO and H and contain a zinc atom in the active sites essential for catalysis. There are many isoforms of CA in mammals and they all belong to the α class (1,2). CA12, a member of the α class, is a plasma membrane protein with the catalytic domain in the extracellular space. CA12 is widely expressed in many tissues and is found at high levels in renal cell cancers. Its expression is induced by hypoxia in solid tumors and is considered to be a tumor marker (3-5). Genetic mutations in CA12 are associated with hyperchlorhidrosis (6,7).
Alternate Names
CA-XII; CA12; CAH12; Carbonate dehydratase XII; Carbonic anhydrase 12; Carbonic anhydrase XII; carbonic dehydratase; CAXII; FLJ20151; HsT18816; T18816; Tumor antigen HOM-RCC-3.1.3
