Description
Product Usage Information
Western Blotting: 1:1000
Immunoprecipitation: 1:50
Immunofluorescence (Immunocytochemistry): 1:400
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 75723 .
Protocol
Available protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Immunocytochemistry)
Specificity / Sensitivity
PSA/KLK3 (D2A8) Rabbit Monoclonal Antibody recognizes endogenous levels of total PSA/KLK3 protein.
Species Reactivity: Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Phe110 of human PSA/ KLK3 protein.
Background
Kallikrein 3 (KLK3), also known as Prostate Specific Antigen (PSA), is a member of the glandular kallikrein subfamily of serine proteases (1). It is produced by prostate epithelial cells and is secreted into prostatic ducts. Upon cleavage of 7 amino-terminal amino acids (2), it is activated to liquefy semen in the seminal coagulum. Although PSA/KLK3 is produced in healthy individuals, investigators have found abnormally high levels in the blood of men with advanced prostate cancer (2,3).
Alternate Names
APS; Gamma-seminoprotein; hK3; kallikrein related peptidase 3; Kallikrein-3; kallikrein-related peptidase 3; KLK2A1; KLK3; P-30 antigen; prostate specific antigen; Prostate-specific antigen; PSA; Semenogelase; Seminin
