Description
Product Usage Information
Western Blotting: 1:1000
Immunoprecipitation: 1:50
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting, Immunoprecipitation
Specificity / Sensitivity
Mineralocorticoid Receptor (E9W1M) Rabbit Monoclonal Antibody recognizes endogenous levels of total mineralocorticoid receptor protein.
Species Reactivity: Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of human mineralocorticoid receptor protein.
Background
Mineralocorticoid receptor (MR) is a steroid hormone receptor with structural and functional similarities to glucocorticoid receptor (GR). MR binds with high affinity to aldosterone and other mineralocorticoids as well as glucocorticoids (1-3). Upon ligand binding, MR undergoes conformational changes and enters the nucleus to bind to target mineralocorticoid response elements (MREs) (3-5). MR is also able to heterodimerize with GR and bind to hormone response elements on DNA in cells that express both receptors (6-8). Unlike the anti-inflammatory activity of GR, aldosterone and MR can promote cardiovascular inflammation (9,10). Since MR can utilize both aldosterone and glucocorticoids as ligands, selectivity of ligands is facilitated by tissue-specific expression of 11 beta-hydroxysteroid dehydrogenase (11 beta-OHSD). This enzyme converts cortisol to cortisone, a derivative with very little affinity to MR, thus providing a means for tissue-specific MR regulation (11,12). Deficiency of 11 beta-OHSD has shown to cause an excess of MR signaling resulting in hypertension (12,13). Antimineralocorticoid spirolactones have been used as synthetic steroids to induce a transcriptionally inactivated conformational change in MR to treat hypertension and sodium retention (14,15).
Alternate Names
aldosterone receptor; FLJ41052; MCR; MGC133092; Mineralocorticoid receptor; mineralocorticoid receptor 1; mineralocorticoid receptor 2; mineralocorticoid receptor delta; MLR; MR; NR3C2; NR3C2VIT; Nuclear receptor subfamily 3 group C member 2; nuclear receptor subfamily 3, group C, member 2; nuclear receptor subfamily 3, group C, member 2 variant 3
