Description
Product Usage Information
Western Blotting: 1:1000
Immunoprecipitation: 1:100
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting, Immunoprecipitation
Specificity / Sensitivity
RTN3 (F5X9D) Rabbit Monoclonal Antibody recognizes endogenous levels of total RTN3 protein.
Species Reactivity: Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human RTN3 protein.
Background
Reticulon-3 (RTN3), a member of the reticulon protein family, plays a crucial role in maintaining the tubular shape and structural integrity of the endoplasmic reticulum (ER) network (1,2). The longest isoform of RTN3 (Rtn3L) carries six active LC3-interacting region domains, which are essential for LC3/GABARAP binding, fragmentation of ER tubules, and its function as an ER-phagy receptor (3,4). Rtn3L is also recruited to ER-endosome membrane contact sites by the endosomal protein Rab9a, where it promotes endosome maturation and cargo sorting (5). RTN3 is highly expressed in neuronal tissues and is a negative modulator of β-amyloid converting enzyme 1 (BACE1), the enzyme that cleaves amyloid precursor protein (APP) to release β-amyloid peptides (6). Therefore, altered expression of RTN3 may modulate amyloid plaque formation, a hallmark of Alzheimer's disease (7,8).
Alternate Names
ASY interacting protein; ASYIP; HAP; homolog of ASY protein; isoforme III; isoforme VI; Neuroendocrine-specific protein-like 2; Neuroendocrine-specific protein-like II; NSP-like protein 2; NSP-like protein II; NSPL2; NSPLII; reticulon 3; Reticulon-3; RTN3; RTN3-A1
