Description
Product Usage Information
Immunohistochemistry (Paraffin): 1:100 - 1:400
Flow Cytometry (Fixed/Permeabilized): 1:200 - 1:800
Flow Cytometry (Live): 1:200 - 1:800
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Immunohistochemistry (Paraffin), Flow Cytometry (Fixed/Permeabilized), Flow Cytometry (Live)
Specificity / Sensitivity
PIGR (F9A8F) Rabbit Monoclonal Antibody recognizes endogenous levels of total PIGR protein. This antibody reacts with PIGR and secretory component (SC).
Species Reactivity: Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val28 of human PIGR protein.
Background
Polymeric immunoglobulin receptor (PIGR) is a highly glycosylated type I membrane protein that mediates transcytosis of immunoglobulin A (IgA) and immunoglobulin M (IgM) across epithelial cells at mucosal surfaces (1,2). The extracellular portion of PIGR is made up of six domains. Domains 1 through 5 are composed of Ig-like domains that bind to IgA dimers or IgM pentamers, and domain 6 contains a conserved proteolytic cleavage site (3). PIGR binds Ig made in the lamina propria via the joining (J) chain. It undergoes clathrin-mediated endocytosis and is further transcytosis through the epithelial cell to the mucosa (4). PIGR then undergoes endoproteolytic cleavage and disassociates from the membrane domain to form the secretory component (SC) and associated Ig, or secretory Ig (SIg) (5). SC enhances SIg stability by resisting proteolytic degradation and can act as a non-specific microbial scavenger, leading to the sequestration of bacteria in the mucus layer (6,7). PIGR expression and SIg secretion are induced by microbial factors, including LPS, butyrate, and dsRNA, and by pro-inflammatory cytokines like IFN-γ, TNF-α, IL-1, and IL-17 (8). PIGR is expressed aberrantly in cancers and has been proposed as a biomarker with prognostic value and as a therapeutic target (9-11).
Alternate Names
FLJ22667; hepatocellular carcinoma associated protein TB6; Hepatocellular carcinoma-associated protein TB6; MGC125361; MGC125362; PIGR; Poly-Ig receptor; Polymeric immunoglobulin receptor; Secretory component
